Separation technique in Chromatography
The type of interaction between
stationary phase, mobile phase, and substances contained in the mixture is the
basic component effective on separation of molecules from each other.
Chromatography methods based on partition are very effective on separation, and
identification of small molecules as amino acids, carbohydrates, and fatty
acids. However, affinity chromatographers (i.e. ion-exchange chromatography)
are more effective in the separation of macromolecules as nucleic acids, and
proteins. Paper chromatography is used in the separation of proteins, and in
studies related to protein synthesis; gas-liquid chromatography is utilized in
the separation of alcohol, Esther, lipid, and amino groups, and observation of enzymatic
interactions, while molecular-sieve chromatography is employed especially for
the determination of molecular weights of proteins. Agarose-gel chromatography
is used for the purification of RNA, DNA particles, and Virus.