Partition chromatography

In Partition chromatography, the molecules are separated in between two phases i.e. both stationary phase and mobile phase are in same phase. The process of separating mixtures of chemical compounds by passing them through a column that contained a solid stationary phase that was eluted with a mobile phase (column chromatography) was well known at that time.


  • Used for final purification natural extracts
  • Synthetic mixtures and biological matrices
  • It is also used for fractionization of complex crude extracts e.g.. Petroleum fractions
  • Determination of water quality
  • Separation of aroma molecules of wine

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