Ion exchange chromatography

The most popular method for the purification of proteins and other charged molecules is ion exchange chromatography.This type of chromatography is additionally subdivided into cation exchange chromatography and anion-exchange chromatography. The ionic compound comprising of the cationic species M+ and the anionic species B-can be held by the stationary phase.Conversely, in anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support. In cation exchange chromatography positively charged particles are pulled in to a contrarily charged solid support. Then again, in anion exchange chromatography, negatively charged atoms are attracted in to a positively charged solid support.

               Anion exchange chromatography
               Cation exchange chromatography
               Principles involved in Ion exchange chromatography
               Ion exchange chromatography considerations

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